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STARTLE AND FEAR COMBINED SYSTEM

The StartFear Combined system is a polyvalent system for conducting both fear conditioning and startle reflex experiments in one same enclosure, regardless the animal is a rat or a mouse (from 15grs to 500 grs.).

Basically, the StartFear system allows recording and analysis of the signal generated by the animal movement through a high sensitivity Weight Transducer system.

The analogical signal is transmitted to the FREEZING and STARTLE software modules through the load cell unit for recording purposes and posterior analysis in terms of activityimmobility (FREEZING) or startle response characterization (STARTLE).

An aditional interface associated with corresponding hardware allows controlling the stimuli (light, sounds, shock, air puff) from the STARTLE and FREEZING software modules.

The StartFear cage is made with black methacrylate walls and a transparent front door. In fear conditioning experiment, the walls, cover and floor can be of different materials or colours. Moreover, a transparent cylinder can be placed into the experimental chamber in order to modify the contextual spatial perception of the subject during the test phase.
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SPATIAL PLACE PREFERENCE BOX

The Panlab spatial place preference box is an experimental chamber developed with the aim to optimise place preference and aversion studies in small laboratory animals, especially mice. The design of the box is fruit of a close collaboration with eminent Professor Dr. Rafael Maldonado and Dr. Olga Valverde from the Laboratory of Neuropharmacology in Barcelona (Spain).

The apparatus consists in a box with two equally sized compartments interconnected by a rectangular corridor. Originally, the compartments are differentiated by the motifs painted on the walls (dots or stripes) and the colour (different shade of grey tones, light or dark) and texture (smooth or rough) of the floor. The innovation brought by the Panlab box is the possibility to combine a new additional spatial dimension to allow the animal differentiate the different compartments in a more discriminative manner. Transparent walls are also used to minimize the time the animal spent in the corridor.

The introduction of these new discriminative elements allows:
- optimising the results obtained in the place preference and aversion paradigms (low variability in the response, reduced number of animals per group.)
- organizing the discriminative elements in a wide variety of configurations for studies evaluating spatial or contextual memory
- using the elements as discriminative cues associated with drug exposure in diverse other experimental designs.

The Panlab spatial place preference box can be associated with the weigth transducer technology (and PPC win software) or video-tracking system (SMART software) for detection and analysis of animal position throughout the test.
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SELF-ADMINISTRATION BOX

The PANLAB self-administration box is an entirely modular experimental enclosure designed to conduct a wide variety of different schedules for studying reward and addiction in laboratory animals.

The chamber is assembled with black aluminium walls and a transparent front door. The chambers employ a stainless-steel grid floor that allows waste to collect in a removable tray. All floor components (including the grid) are removable for systematic cleaning.

Special modules are available for self-administration and self-stimulation procedures: lever or nose-spoke, food or drink dispensers, drug delivery system and stimuli (light, sound, shock). Each chamber is associated with a Link Box which provides power to up to 8 (expandable to 16) self-administration Modules conferring to the chambers a full autonomy. Only one cable connects the Link Box to the PC, this last for advanced protocol configuration and running.

All Panlab self-administration boxes are associated with the potent and versatile Packwin software which allows configuring any kind of user-defined schedules (training, priming, fixed-ratio, progressive ratio, extinction, relapse etc) and providing relevant data in this context (number of pressing on active and inactive levers, number of injection received, pattern response graph etc)
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Rotameter

Rotational behaviour has proved a popular technique for screening the behavioural effects of a wide variety of lesions, drugs, and other experimental manipulations on the brain of rodents. This test is widely carried out in experiments using animal models of Parkinson disease with unilateral lesions in the dopaminergic nigrostriatal system.

Basically, the subject wears an adjustable harness with a soft ring connected to the rotation sensor by a flexible tie. The subject is then placed into a transparent container (cylinder or bowl) with a lateral support for a vertical stand.

A bi-directional rotation sensor provides a double (right and left turns) output with adjustable regulation of pulsesturns. Two configurations are available for rotation counting: individual counter or a multicounter.

When using a multicounter, the new SEDACOM 2.0 version provides an easy and convenient way to visualize and export the data on a computer for further analysis.
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Tail-Flick Meter

  • Voltage 50W-12V
Stimulus is provided by a halogen lamp (50W-12V) set on a pivoting stand.
A bright beam shows the point on which the heat source will focus.
The lamp has 99 heat intensities to cover a temperature gradient of 40C to 220C in 10s
Can be used on animals weighting 20g to 400g.
The response latency is measured with 0.01 sec resolution.
Manual and remote operation. Pedal included.
Safety device which can be adjusted by the user to the maximum time of response. ( factory adjustment: 20 sec).
Equipped with RS232 port. Includes SeDaCom software for transmitting the data to the PC in ASCI format.
Supplied with 250-g restrainer.
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Thermal Place Preference

As advised by A. MOQRICH, and published in Moqrich et al (Science 2005, 307: 1468-72), this Thermal Place Preference Test allows researcher to work on unrestrained animals (mice and rats) let free to choose their preferred position (comfort zone) between 2 compartments set at different temperatures. This behavioural assay will allow monitoring temperature preferences, nociceptive thresholds and state in the role of a given gene or a compound on these pain thresholds associated to cold and hot stimulation.

This Temperature Choice Test from Bioseb is investigator-independent, unlike the cold andor hot plate test: using the traditional plates, an operator can measure the reaction time of an animal (mouse or rat) exposed to a certain temperature. The "two Temperatures Choice Test" will return a nociceptive response without any action from the operator, and the obtained value is a temperature or a temperature range indicating the sensitivity of the animal (mouse or rat) resulting to the exposure to different stimulations (cold or heat).
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Coverslip/Coverglass

Cover glasses with diameters of 5 mm and 8 mm are now available from stock in convenient packages of 100.
Available in #1.0 (0.15 mm), #1.5 (0.17 mm) and #2.0 (0.22 mm) thickness
Available in round, square and rectangular geometries
New 3 mm round for cranial window technique
Stocked in depth for fast delivery
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Pe Tubing

  • Exhibitors Warner
We stock the sizes compatible with our chambers and other perfusion devices. Consult the specifications chart to select the proper size tubing for your perfusion device.
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Electronic Von-Frey

  • Weight 6, 5kg
  • Measurement range 0 to 500 g (5N)
Key Features: Provides objective and accurate data The threshold value can be obtained in only one test, and in a highly reproducible manner Elimination of the problems of fi lament standardizat
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Silicone Grease Kit

An artist's acrylic brush is an effective tool for applying silicone lubricant to a glass coverslip and polycarbonate chamber. This kit greatly simplifies the assembly of a Warner Recording and Perfusion Chambers.
This convenient package includes a tube of Dow Corning 111 Valve Lubricant & Sealant, two acrylic paint brushes (sized #2 and #4), and several pallets. By "painting" the grease onto the bottom surface of a polycarbonate chamber, it is easy to evenly spread lubricant and create a water-tight seal.
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  • Lillian Zhou (DL Naturegene Life Sciences)
  • 587 N Ventu Park Road, Suite E535 Newbury Park, AC 91320, United States
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