Reagen Llc

New Jersey, United States

Product Description REAGEN™ Methyltestosterone ELISA Test Kit provides a competitive enzyme immunoassay for the quantitative analysis of methyltestosteronein feed, fish, shrimp, meat and urine. The unique features of the kit are:  Rapid (10-40min), high recovery (>80%), and cost-effective extraction methods .  High sensitivity (0.1 ng/g or ppb) and low detection limit (1 ng/g or ppb) .  A quick ELISA assay (less than 2 hours regardless of number of samples).  High reproducibility. Procedure Overview The method is based on a competitive colorimetric ELISA assay. The drug of interest has been coated in the plate wells. During the analysis, sample is added along with the primary antibody specific for the target drug. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the drug attached to the well. The secondary antibody, tagged with a peroxidase enzyme, targets the primary antibody that is complexed to the drug coated on the plate wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample. Kit Contents, Storage and Shelf Life REAGEN™ Methyltestosterone ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8C*. The shelf life is 12 months when the kit is properly stored. Kit Contents Amount Storage Methyltestosterone-coated Microtiter Plate 96well plate(8wells x12 strips) 2-8C Methyltestosterone Standards: Negative control (white cap tube) 0.1ng/mL (yellow cap tube) 0.5ng/mL (orange cap tube) 2.5 ng/mL (pink cap tube) 7.5 ng/mL (purple cap tube) 25 ng/mL (blue cap tube) 100 ng/mL (spiking, optional, red cap tube) 0.8 mL 0.8 mL 0.8 mL 0.8 mL 0.8 mL 0.8 mL 0.8 mL 2-8C Methyltestosterone Antibody #1 15 mL 2-8C * 100X HRP-Conjugated Antibody #2 250L 2-8C * Antibody #2 Diluent ** 20 mL 2-8C 10X Sample Extraction Buffer ** 25 mL 2-8C 20X Wash Solution ** 28 mL 2-8C Stop Buffer ** 20 mL 2-8C TMB Substrate ** 12 mL 2-8C Concentrate of Feed Extraction Buffer 60g 2-8C Feed Clean Up Mix 12g 2-8C * If you are not planning to use the kit for over 3 months, store Antibody #1 and 100X HRP-Conjugated Antibody #2 at -20C or in a freezer. Sensitivity Sample Type Detection Limit (ng/g or ppb) Feed 0.1 Shrimp/Fish/Meat 1 Urine 0.5

Product Description REAGEN™Trenbolone ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Trenbolone in fish, shrimp, meat and urine. The unique features of the kit are:  High recovery (>80%), rapid(10-40 minutes), and cost-effective extraction methods.  High sensitivity(0.05 ppb)and low detection limit(0.025ppb) for meat sample  A quick ELISA assay(less than 2 hours regardless of number of samples).  High reproducibility. Procedure Overview The method is based on a competitive colorimetric ELISA assay. The Trenbolone antibody has been coated in the plate wells. During the analysis, sample is added along with the enzyme-conjugate. If the target is present in the sample, it will compete for the antibody, thereby preventing the HRP from binding to the antibody attached to the well.The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample. Kit Contents, Storage and Shelf Life REAGEN™Trenbolone ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C *. The shelf life is 12 months when the kit is properly stored. Kit Contents Amount Storage Trenbolone Coated Plate 1 x 96-well Plate(8 wells x 12 strips) 2-8°C Trenbolone Standards: Negative control (white cap tube) 0.05 ng/mL (yellow cap tube) 0.1 ng/mL (orange cap tube) 0.25 ng/mL (pink cap tube) 0.5 ng/mL (purple cap tube) 1.0 ng/mL (blue cap tube) 10 µg/mL (spiking, optional, red cap tube) 0.8 mL 0.8 mL 0.8 mL 0.8 mL 0.8 mL 0.8 mL 0.8 mL 2-8°C * 180X HRP Conjugate 180 µL 2-8°C * Sample Diluent buffer 20 mLX2 2-8°C 20X Wash Solution 28 mL 2-8°C Stop Buffer 14 mL 2-8°C TMB Substrate 12 mL 2-8°C Sample Balance Buffer A 10 mL 2-8°C Sample Balance Buffer B 62g 2-8°C Tissue Clean Up mix 25g 2-8°C * If you are not planning to use the kit for over 1 month, store Trenbolone Standards, Trenbolone Antibody and 180X HRP Conjugate at -20°C or in a freezer. Sensitivity (Detection Limit) Sample Type Detection Limit (g/g or ppb) Meat/Fish/shrimp 0.025 Urine 0.25