Laboratory Equipments #2569257

Reagenthe botulinus(typeb) polymerase chain reaction(pcr) diagnostic kit is designed to detect the dna of botulinus(type b) in the feces, water and serum sample.but the result is only advisory.

procedure overview
reagenthe botulinus(typeb) polymerase chain reaction(pcr) diagnostic kit extract dna with a pair of botulinum (bl) b type specific primers and a specificity of fluorescent probe. After extracting dna, and the latter in the role of taq dna polymerase, match with fq - buffer (containing magnesium 2 +, tris - hcl, etc.), four kinds of nucleotide monomers (dntps), and other elements, through the pcr - fluorescent probes in vitro amplification method for botulinum type (bl) b dna in vitro amplification, so as to achieve rapid detection of clostridium botulinum type (bl) b.

kit contents, storage and shelf life

kit contents amount storage
nucleic acid extraction buffer 2ml -20c
taq dna polymerase 50l -20c
bl-b-qpcr mix 1ml -20c
bl-b -positive control 50l -20c
negative control 250l -20c
the kits specifications is 48 reactions/box, the validity of this kit is six months , should be preserved in - 20 , avoid repeated freezing and thawing.

required materials
pcr instrument with double or more channels.

sample collection and storage and transportation

1. Human or animal feces
collect fresh feces with mucus purulent blood, sealed samples, then test.
2. Blood
collect fresh anticoagulant fresh blood (non heparin anticoagulation), sealed samples, then test.

dna extraction

feces
1. take feces sample to a centrifuge tube which contains 0.5ml saline, vortex for 1min at maximum speed.centrifuge for 10 minutes at 13, 000 x g at room temperature (20 25c).
2. discard the supernatant and add 100l dna extraction buffervortex for 30s at maximum speed.then heat to boil by electric furnace , stir 10min. Centrifuge for 5 minutes at 13, 000 x g at room temperature (20 25c).use the 4l supernatant for the assay.

water
1. take 3ml water sample and centrifuge for 2 minutes at 13, 000 x g at room temperature (20 25c).
2. discard the supernatant and add 100l dna extraction buffervortex for 30s at maximum speed.then heat to boil by electric furnace , stir 10min. Centrifuge for 5 minutes at 13, 000 x g at room temperature (20 25c

REAGENThe botulinus(TypeE/F) polymerase chain reaction(PCR) diagnostic kit is designed to detect the DNA of botulinus(type E/F) in the feces, water and serum sample.But the result is only advisory.

Procedure Overview
REAGENThe botulinus(TypeE/F) polymerase chain reaction(PCR) diagnostic kit extract DNA with a pair of botulinum (BL) E/F type specific primers and a specificity of fluorescent probe. After extracting DNA, and the latter in the role of Taq DNA polymerase, match with FQ - Buffer (containing magnesium 2 +, Tris - HCl, etc.), four kinds of nucleotide monomers (dNTPs), and other elements, through the PCR - fluorescent probes in vitro amplification method for botulinum type (BL) E/F DNA in vitro amplification, so as to achieve rapid detection of clostridium botulinum type (BL) E/F.

Kit Contents, Storage and Shelf Life

Kit Contents Amount Storage
Nucleic acid extraction buffer 2mL -20C
Taq DNA Polymerase 50L -20C
BL-E/F-qPCR MIX 1mL -20C
BL-E/F -positive control 50L -20C
Negative control 250L -20C
The kits specifications is 48 reactions/box, the validity of this kit is six months , should be preserved in - 20 , avoid repeated freezing and thawing.

Required Materials
PCR instrument with double or more channels.

Sample collection and storage and transportation

1. Human or animal feces
Collect fresh feces with mucus purulent blood, sealed samples, then test.
2. Blood
Collect fresh anticoagulant fresh blood (non heparin anticoagulation), sealed samples, then test.

Reagenthe botulinus(typea) polymerase chain reaction(pcr) diagnostic kit is designed to detect the dna of botulinus(type a) in the feces, water and serum sample.but the result is only advisory.

procedure overview
reagenthe botulinus(typea) polymerase chain reaction(pcr) diagnostic kit extract dna with a pair of botulinum (bl) a type specific primers and a specificity of fluorescent probe. After extracting dna, and the latter in the role of taq dna polymerase, match with fq - buffer (containing magnesium 2 +, tris - hcl, etc.), four kinds of nucleotide monomers (dntps), and other elements, through the pcr - fluorescent probes in vitro amplification method for botulinum type (bl) a dna in vitro amplification, so as to achieve rapid detection of clostridium botulinum type (bl) a.

kit contents, storage and shelf life
kit contents amount storage
nucleic acid extraction buffer 2ml -20c
taq dna polymerase 50l -20c
bl-a-qpcr mix 1ml -20c
bl-a -positive control 50l -20c
negative control 250l -20c
the kits specifications is 48 reactions/box, the validity of this kit is six months , should be preserved in - 20 , avoid repeated freezing and thawing.

required materials
pcr instrument with double or more channels.

sample collection and storage and transportation

1. Human or animal feces
collect fresh feces with mucus purulent blood, sealed samples, then test.
2. Blood
collect fresh anticoagulant fresh blood (non heparin anticoagulation), sealed samples, then test.

Reagenthe botulinus(typea/b) polymerase chain reaction(pcr) diagnostic kit is designed to detect the dna of botulinus(type a/b) in the feces, water and serum sample.but the result is only advisory.

procedure overview
reagenthe botulinus(typea/b) polymerase chain reaction(pcr) diagnostic kit extract dna with a pair of botulinum (bl) a/b type specific primers and a specificity of fluorescent probe. After extracting dna, and the latter in the role of taq dna polymerase, match with fq - buffer (containing magnesium 2 +, tris - hcl, etc.), four kinds of nucleotide monomers (dntps), and other elements, through the pcr - fluorescent probes in vitro amplification method for botulinum type (bl) a/b dna in vitro amplification, so as to achieve rapid detection of clostridium botulinum type (bl) a/b.

kit contents, storage and shelf life
kit contents amount storage
nucleic acid extraction buffer 2ml -20c
taq dna polymerase 50l -20c
bl-a/b-qpcr mix 1ml -20c
bl-a/b -positive control 50l -20c
negative control 250l -20c
the kits specifications is 48 reactions/box, the validity of this kit is six months , should be preserved in - 20 , avoid repeated freezing and thawing.


required materials
pcr instrument with double or more channels.

sample collection and storage and transportation

1. Human or animal feces
collect fresh feces with mucus purulent blood, sealed samples, then test.
2. Blood
collect fresh anticoagulant fresh blood (non heparin anticoagulation), sealed samples, then test.

dna extraction

Basicparametersofinstrument
weight: 8.1kg
overalldimensions: 430mml335mmw175mmh
power: a.c.110v220v50-60hz
fuses: t2al250v
workenvironment: temperature10-30humidity70%
storeenvironment: -20-55humidity93%
standardwavelength: 405450492630nmtenopticalfiberscanbeconfiguredwithin400to800atatime;
instrumentsensitivity: nolessthan0.01lmg.
Instrumentchanneldifference: lessthan0.01a.
Rangeofoperatingwavelength: 400-800nm;

detectionrange: 0.000-4.000a;
resolutionratio: 0.001a;
platereadingvelocity: 5sofsinglewaveform, 10sofdualwavelength;
warmuptime: 10minute
interface: rs-232cserialinterface, usbinterface
display: 5.7lcddisplay320240discernibility, 256grayscale
input: touchpanelandpen,
technicalspecifications
1)wavelengthcharacteristics:requirementsonaccuratewavelengthandhalfwavewidthofopticalfibersforinstrumentseethetablebelow.
Unit:nm
accuratewavelength halfwavewidth
3.0 12
2)accurateabsorbanceandlinearerror:measurementaccuracyandlinearerrorofinstrumentshallcomplywiththeprovisionsasdescribedinthetablebelow:
absorbancerange accuracy linearerror
0.000-2.000 0.01 1.0%
>2.000-3.000 0.05 2.0%
>3.000-4.000 0.15 5.0%
3)repeatabilityofmeasurementofinstrument:nogreaterthan0.5%.
4)instrumentstability:
a)absorbancevariesshallbenogreaterthan0.005awithin10minduetodrifting.
B)ifthepowersupplyvoltageof220vvariesby10%, theresultingvariationofabsorbanceisnogreaterthan0.005a.