Reagenllc

Moorestown, United States

Reagenthe botulinus(typeb) polymerase chain reaction(pcr) diagnostic kit is designed to detect the dna of botulinus(type b) in the feces, water and serum sample.but the result is only advisory. procedure overviewreagenthe botulinus(typeb) polymerase chain reaction(pcr) diagnostic kit extract dna with a pair of botulinum (bl) b type specific primers and a specificity of fluorescent probe. After extracting dna, and the latter in the role of taq dna polymerase, match with fq - buffer (containing magnesium 2 +, tris - hcl, etc.), four kinds of nucleotide monomers (dntps), and other elements, through the pcr - fluorescent probes in vitro amplification method for botulinum type (bl) b dna in vitro amplification, so as to achieve rapid detection of clostridium botulinum type (bl) b. kit contents, storage and shelf life kit contents amount storagenucleic acid extraction buffer 2ml -20ctaq dna polymerase 50l -20cbl-b-qpcr mix 1ml -20cbl-b -positive control 50l -20cnegative control 250l -20cthe kits specifications is 48 reactions/box, the validity of this kit is six months , should be preserved in - 20 , avoid repeated freezing and thawing. required materialspcr instrument with double or more channels. sample collection and storage and transportation 1. Human or animal fecescollect fresh feces with mucus purulent blood, sealed samples, then test. 2. Bloodcollect fresh anticoagulant fresh blood (non heparin anticoagulation), sealed samples, then test. dna extraction feces1. take feces sample to a centrifuge tube which contains 0.5ml saline, vortex for 1min at maximum speed.centrifuge for 10 minutes at 13, 000 x g at room temperature (20 25c). 2. discard the supernatant and add 100l dna extraction buffervortex for 30s at maximum speed.then heat to boil by electric furnace , stir 10min. Centrifuge for 5 minutes at 13, 000 x g at room temperature (20 25c).use the 4l supernatant for the assay. water1. take 3ml water sample and centrifuge for 2 minutes at 13, 000 x g at room temperature (20 25c). 2. discard the supernatant and add 100l dna extraction buffervortex for 30s at maximum speed.then heat to boil by electric furnace , stir 10min. Centrifuge for 5 minutes at 13, 000 x g at room temperature (20 25c

REAGENThe botulinus(TypeE/F) polymerase chain reaction(PCR) diagnostic kit is designed to detect the DNA of botulinus(type E/F) in the feces, water and serum sample.But the result is only advisory. Procedure OverviewREAGENThe botulinus(TypeE/F) polymerase chain reaction(PCR) diagnostic kit extract DNA with a pair of botulinum (BL) E/F type specific primers and a specificity of fluorescent probe. After extracting DNA, and the latter in the role of Taq DNA polymerase, match with FQ - Buffer (containing magnesium 2 +, Tris - HCl, etc.), four kinds of nucleotide monomers (dNTPs), and other elements, through the PCR - fluorescent probes in vitro amplification method for botulinum type (BL) E/F DNA in vitro amplification, so as to achieve rapid detection of clostridium botulinum type (BL) E/F. Kit Contents, Storage and Shelf Life Kit Contents Amount StorageNucleic acid extraction buffer 2mL -20CTaq DNA Polymerase 50L -20CBL-E/F-qPCR MIX 1mL -20CBL-E/F -positive control 50L -20CNegative control 250L -20CThe kits specifications is 48 reactions/box, the validity of this kit is six months , should be preserved in - 20 , avoid repeated freezing and thawing. Required MaterialsPCR instrument with double or more channels. Sample collection and storage and transportation 1. Human or animal fecesCollect fresh feces with mucus purulent blood, sealed samples, then test.2. BloodCollect fresh anticoagulant fresh blood (non heparin anticoagulation), sealed samples, then test.

Reagenthe botulinus(typea) polymerase chain reaction(pcr) diagnostic kit is designed to detect the dna of botulinus(type a) in the feces, water and serum sample.but the result is only advisory. procedure overviewreagenthe botulinus(typea) polymerase chain reaction(pcr) diagnostic kit extract dna with a pair of botulinum (bl) a type specific primers and a specificity of fluorescent probe. After extracting dna, and the latter in the role of taq dna polymerase, match with fq - buffer (containing magnesium 2 +, tris - hcl, etc.), four kinds of nucleotide monomers (dntps), and other elements, through the pcr - fluorescent probes in vitro amplification method for botulinum type (bl) a dna in vitro amplification, so as to achieve rapid detection of clostridium botulinum type (bl) a. kit contents, storage and shelf lifekit contents amount storagenucleic acid extraction buffer 2ml -20ctaq dna polymerase 50l -20cbl-a-qpcr mix 1ml -20cbl-a -positive control 50l -20cnegative control 250l -20cthe kits specifications is 48 reactions/box, the validity of this kit is six months , should be preserved in - 20 , avoid repeated freezing and thawing. required materialspcr instrument with double or more channels. sample collection and storage and transportation 1. Human or animal fecescollect fresh feces with mucus purulent blood, sealed samples, then test. 2. Bloodcollect fresh anticoagulant fresh blood (non heparin anticoagulation), sealed samples, then test.

Reagenthe botulinus(typea/b) polymerase chain reaction(pcr) diagnostic kit is designed to detect the dna of botulinus(type a/b) in the feces, water and serum sample.but the result is only advisory. procedure overviewreagenthe botulinus(typea/b) polymerase chain reaction(pcr) diagnostic kit extract dna with a pair of botulinum (bl) a/b type specific primers and a specificity of fluorescent probe. After extracting dna, and the latter in the role of taq dna polymerase, match with fq - buffer (containing magnesium 2 +, tris - hcl, etc.), four kinds of nucleotide monomers (dntps), and other elements, through the pcr - fluorescent probes in vitro amplification method for botulinum type (bl) a/b dna in vitro amplification, so as to achieve rapid detection of clostridium botulinum type (bl) a/b. kit contents, storage and shelf lifekit contents amount storagenucleic acid extraction buffer 2ml -20ctaq dna polymerase 50l -20cbl-a/b-qpcr mix 1ml -20cbl-a/b -positive control 50l -20cnegative control 250l -20cthe kits specifications is 48 reactions/box, the validity of this kit is six months , should be preserved in - 20 , avoid repeated freezing and thawing. required materialspcr instrument with double or more channels. sample collection and storage and transportation 1. Human or animal fecescollect fresh feces with mucus purulent blood, sealed samples, then test. 2. Bloodcollect fresh anticoagulant fresh blood (non heparin anticoagulation), sealed samples, then test. dna extraction

Basicparametersofinstrumentweight: 8.1kgoveralldimensions: 430mml335mmw175mmhpower: a.c.110v220v50-60hzfuses: t2al250vworkenvironment: temperature10-30humidity70%storeenvironment: -20-55humidity93%standardwavelength: 405450492630nmtenopticalfiberscanbeconfiguredwithin400to800atatime;instrumentsensitivity: nolessthan0.01lmg.Instrumentchanneldifference: lessthan0.01a.Rangeofoperatingwavelength: 400-800nm; detectionrange: 0.000-4.000a;resolutionratio: 0.001a;platereadingvelocity: 5sofsinglewaveform, 10sofdualwavelength;warmuptime: 10minuteinterface: rs-232cserialinterface, usbinterfacedisplay: 5.7lcddisplay320240discernibility, 256grayscaleinput: touchpanelandpen, technicalspecifications1)wavelengthcharacteristics:requirementsonaccuratewavelengthandhalfwavewidthofopticalfibersforinstrumentseethetablebelow.Unit:nmaccuratewavelength halfwavewidth3.0 122)accurateabsorbanceandlinearerror:measurementaccuracyandlinearerrorofinstrumentshallcomplywiththeprovisionsasdescribedinthetablebelow:absorbancerange accuracy linearerror0.000-2.000 0.01 1.0%>2.000-3.000 0.05 2.0%>3.000-4.000 0.15 5.0%3)repeatabilityofmeasurementofinstrument:nogreaterthan0.5%.4)instrumentstability:a)absorbancevariesshallbenogreaterthan0.005awithin10minduetodrifting.B)ifthepowersupplyvoltageof220vvariesby10%, theresultingvariationofabsorbanceisnogreaterthan0.005a.